Silencing CAFFEOYL SHIKIMATE ESTERASE Affects Lignification and Improves Saccharification in Poplar1[OPEN]
نویسندگان
چکیده
Marina de Lyra Soriano Saleme , Igor Cesarino, Lívia Vargas, Hoon Kim, Ruben Vanholme, Geert Goeminne, Rebecca Van Acker, Fernando Campos de Assis Fonseca, Andreas Pallidis, Wannes Voorend, José Nicomedes Junior , Dharshana Padmakshan, Jan Van Doorsselaere, John Ralph, and Wout Boerjan Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium VIB Center for Plant Systems Biology, 9052 Ghent, Belgium Department of Botany, Institute of Biosciences, University of São Paulo, 05508-090 Butanta, Sao Paulo, Brazil Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726 Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706 Petróleo Brasileiro S.A., Centro de Pesquisas Leopoldo Américo Miguez de Mello, Rio de Janeiro, 21941-598, Brazil VIVES, 8800 Roeselare, Belgium
منابع مشابه
Silencing CAFFEOYL SHIKIMATE ESTERASE Affects Lignification and Improves Saccharification in Poplar.
Caffeoyl shikimate esterase (CSE) was recently shown to play an essential role in lignin biosynthesis in Arabidopsis (Arabidopsis thaliana) and later in Medicago truncatula However, the general function of this enzyme was recently questioned by the apparent lack of CSE activity in lignifying tissues of different plant species. Here, we show that down-regulation of CSE in hybrid poplar (Populus ...
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BACKGROUND Caffeoyl shikimate esterase (CSE) was recently characterized as an enzyme central to the lignin biosynthetic pathway in Arabidopsis thaliana. The cse-2 loss-of-function mutant shows a typical phenotype of lignin-deficient mutants, including collapsed vessels, reduced lignin content, and lignin compositional shift, in addition to a fourfold increase in cellulose-to-glucose conversion ...
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Cell wall hemicelluloses and pectins are O-acetylated at specific positions, but the significance of these substitutions is poorly understood. Using a transgenic approach, we investigated how reducing the extent of O-acetylation in xylan affects cell wall chemistry, plant performance and the recalcitrance of lignocellulose to saccharification. The Aspergillus niger acetyl xylan esterase AnAXE1 ...
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